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Introduction to Biology - The Secret of Life
Introduction to Biology - The Secret of Life
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信息技术2017级一班
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6.18How to Edit a Gene
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1.1Week 1 Introduction
1.2Medical Revolutions in Biology
1.3Biological applications in forensics, history, and agriculture
1.4The diversity of life
1.5An overview of evolution and a comparison of prokaryotic and eukaryotic cells
1.6The fundamental principles and intellectual framework of biology
1.7Fractionating life
1.8Molecular composition of cells
1.9Covalent bonds
1.10Non-covalent bonds
1.11Lipids and phospholipids
1.12High energy molecules：ATP and carbohydrates
1.13Reading Chemical Structures
1.14Polarity of Molecules
1.15Intermolecular Bonding
1.16How to Use the Molecule Editor
2.1Week's Introduction
2.2Amazing proteins：primary structure
2.3Meet the amino acids
2.4Amazing proteins：secondary structure
2.5Amazing proteins：tertiary and quaternary structure
2.6Design a channel protein
2.7Enzymes and biochemical reactions
2.8What do enzymes do
2.9How do enzymes work
2.10Influenza virus - tricks of a burglar
2.11The energetics of pathways
2.12Logical tricks of pathways
2.13Glycolysis：a pathway to break down sugar
2.14Regulation of pathways
2.15Cellular respiration and fermentation
2.16Protein Purification - GFP
2.17Protein Purification - Beta-galactosidase
2.18X-ray Structure
2.19How to Explore a Protein
3.1Week's Introduction
3.2Background：Who was Mendel
3.3Mendel's Experiments
3.4Definitions
3.5Multiple traits：Mendel's second law
3.6Cytology and the chromosomal theory of inheritance
3.7Meiosis
3.8Fruit flies and linkage
3.9Linkage Maps
3.10Linkage Mapping
3.11Sex chromosomes and sex linkage
3.12Meiosis and the relationship to Punnett Squares
3.13Meiosis with two genes on different chromosomes
3.14Meiosis with two genes on the same chromosome
3.15Homologous recombination
3.16Modes of Inheritance with Flies
4.1Week's Introduction
4.2X-linked recessive inheritance
4.3Autosomal dominant inheritance
4.4Autosomal recessive inheritance
4.5Real human genetics
4.6Garrod and inborn errors of metabolism
4.7Garrod, Beadle, Tatum and the link between genetics and biochemistry
4.8Yeast as a model organism
4.9How to use genetics to study biochemistry：A mutant hunt
4.10Tricks of a mutant hunt
4.11Characterizing Mutants：Tests of Dominance
4.12Characterizing Mutants：Tests of Complementation
4.13Characterizing mutants：epistasis test
4.14Yeast in the Lab
4.15Pedigree Analysis
5.1Week's Introduction
5.2The Transforming Principle
5.3Structure of DNA∶ Nucleotides and Base-pairing
5.4Bacterial Viruses
5.5DNA Structure, The Race
5.6Meselson and Stahl
5.7Details of DNA Replication
5.8DNA Replication∶ Other Enzymes
5.9DNA Replication∶ Fidelity
5.10Kornberg's Enzyme
5.11Tour of a Nucleotide
5.12DNA Replication
6.1Week's Introduction
6.2RNA
6.3Transcription∶ making RNA copy of DNA
6.4Translation∶ making a polypeptide from RNA
6.5Peering back in time
6.6Replication in different organisms
6.7Transcription in different organisms
6.8Translation in different organisms
6.9Beta-galactosidase in E coli
6.10Lac operon, lactose regulation
6.11Lac operon, glucose regulation
6.12Hormone receptors in mammals
6.13β-globin gene structure
6.14β-globin mutations
6.15The β-globin region
6.16Transcription and Translation
6.17Lac Operon
6.18How to Edit a Gene
7.1Introduction
7.2Overview
7.3Cutting and Pasting DNA
7.4Vectors
7.5Transformation and Selection
7.6Shaggy Dog
7.7Week's Introduction
7.8Tricks for Cloning
7.9Different Cloning Vectors and Source DNA
7.10Finding Your Gene by Complementation
7.11Finding Your Gene by Protein Expression
7.12Gel Electrophoresis
7.13DNA Sequencing, the Concept
7.14DNA Sequencing, Implementation
7.15Polymerase Chain Reaction (PCR)
7.16Lab Video∶ Gel Electrophoresis
7.17Origin of Replication and Promoters
7.18Restriction Enzymes
8.1Weekly Introduction
8.2Recombinant DNA Review
8.3Finding Your Gene∶ Human Mendelian Diseases
8.4Genetic Mapping in Humans
8.5The Human Genome Project∶ Genome assembly and analysis
8.6Improvements since the Human Genome Project
8.7Improvements in DNA Sequencing
8.8The Genomic Landscape
8.9Evolutionary Comparisons
8.10Evolutionary Comparisons as a Tool for Biomedical Research
8.11DNA Polymorphisms within Human Populations
8.12Sanger Sequencing
8.13New Sequencing Technologies
9.1Weekly Introduction
9.2DNA Polymorphisms in Medicine∶ Taking a Comprehensive View
9.3DNA Polymorphisms∶ Polygenic Diseases
9.4RNA Variation
9.5Protein Localization on the Genome
9.6Adding a Gene
9.7Modifying and Subtracting a Gene
9.8RNA Interference
9.9Modern Genome Editing
10.1Weekly Introduction
10.2Heart Disease
10.3Cholesterol
10.4Lipoprotein Particles and the Connection to Heart Disease
10.5The Genetics of Cholesterol Levels
10.6Rational Therapy for Familial Hypercholesterolemia
10.7Modern Strategies
10.8Cancer
10.9Regulation of Cell Growth – Growth Factors and Receptors
10.10Regulation of Cell Growth - Ras
10.11Regulation of Cell Growth – Ras Signaling
10.12Mutations that Cause Cancer
10.13Anti-Cancer Therapy
10.14DNA Fingerprinting
10.15DNA and Law
10.16Other Forensic Technologies
10.17Gene Patenting
10.18Lecture 24 Update
10.19Cancer Biology 1
10.20Cancer Biology 2
11Farewell Message and Credits
12Exam Message from Professor Lander
13Message from Professor Lander
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